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Calcein as a fluorescent iron chemosensor for the - PubMed

The fluorescence quenching of calcein (CA) is not iron specific and results 2.77-3.15 microM range at 100% Tf saturation and 3.09-3.39 microM range at

Fluorescence analysis of the labile iron pool of mammalian cells

We describe here an approach for tracing LIP levels in living cells based on the fluorescent probe calcein (CA). This probe binds Fe(II) rapidly,

Expressed at the Plasma Membrane Transports Iron and

cent, metal-sensitive dye calcein, and a combination of membrane-permeant and -impermeant linear in the range of CoCl2 concentrations tested. CoCl2 in-.

Calcein - an overview | ScienceDirect Topics

Calcein acetoxymethyl (Calcein-AM) is a substrate that passively crosses the s−1 for removal of iron from ferritin by a series of hexadentate ligands

Transport of iron and other transition metals into cells as

of traces of calcein fluorescence quenching. In cases where the slope was not uniform throughout, the longest linear segment of the trace was used.

The role of endocytic pathways in cellular uptake of plasma

Endosomal iron accumulation traced with calcein-green was proportional to input essentially independent of CALG concentration used in the range 5–50μM.

Modulation of iron homeostasis in macrophages by bacterial

A sensitive way to measure the labile iron pool without cell disruption is the use of a membrane permeable fluorescent probe such as calcein

Fluorescent Indicators for Zn2+ and Other Metal Ions—Section

A microplate assay based on the fluorescence quenching of calcein by iron or cadmium in solution, with a linear detection range from 5 to 200 nM. ref

labile iron pool

Clinically interpret test result of a broad range of conventional and more novel iron of deferiprone (def) are linearly dependent on iron concentration.

Chemical tools for detecting Fe ions. - Abstract - Europe PMC

Since iron ions show unique reactivity depending on its redox state, i.e., Fe2+ or Fe3+ (or The first fluorescent probe of Fe ion is calcein.

Non-Invasive Microbial Metabolic Activity Sensing at Single

The fluorescent conversion product of calcein acetoxymethyl ester (CAM) that the regulator of iron homeostasis DtxR controls a range of

Chemical tools for detecting Fe ions. - Abstract - Europe PMC

Since iron ions show unique reactivity depending on its redox state, i.e., Fe2+ or Fe3+ (or The first fluorescent probe of Fe ion is calcein.

The role of endocytic pathways in cellular uptake of plasma

Endosomal iron accumulation traced with calcein-green was proportional to input essentially independent of CALG concentration used in the range 5–50μM.

Structure, fluorescence, and chelating properties of Calcein

flucrochromic indicators, also known as metal 1 of 1 nnT-Asrenr fluorescent species), the monovalent anion (the range of existence of which had never

Preparation, properties and applications of Calcein in a highly

metal-free solutions, Calcein is fluorescent between pH. 5.5 and pH 8.5. excitation and emission spectra over the entire range of pH from 0 to 13.

Mechanism of Effective Quenching of Calcein Fluorescence

PDF | Geometry of the associate of the calcein and iron ion was determined using density functional CAM-B3LYP method.

Iron oxide nanoparticles can cross plasma membranes - Nature

As such, we exposed Calcein-filled oocytes to iron NPs. filled with 3 M KCl, had tip resistances in the 0.5–4 MΩ range.

versión On-line ISSN 0717-9707 - SciELO Chile

Keywords: Mild Steel; Polarization; EIS; Acid Corrosion; Calcein Electrochemical impedance spectroscopy and linear polarization resistance measurements.

Analyzing the Quenchable Iron Pool in Murine Macrophages

Keywords: Quenchable iron pool, Labile iron pool, Cytoplasm, Macrophage, Flow cytometry, Calcein-AM, 8-hydroxyquinoline, FeHQ.

Calcein AM Cell Viability Assay - G-Biosciences

non-fluorescent calcein AM dye (Fig.1) is hydrolyzed by cellular titration cell density curve for linear range and assay suitability for a cell type.